Duolink™ In Situ Red Starter Kit Goat/Rabbit

Stock code :DUO92105
Brand :Sigma-Aldrich
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Duolink™ In Situ Red Starter Kit Goat/Rabbit

Application

Duolinkproximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink PLA Resource Center for information on how to run a Duolink experiment, applications, troubleshooting, and more.

To perform a complete Duolink PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), red detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.

Specificity
The Duolink In Situ Red Starter Kit Goat/Rabbit requires one primary antibody from goat and one primary antibody from rabbit. Red fluorescence detection reagents are often used with Texas Red® filter.

Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

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Features and Benefits

• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results

Legal Information

Duolink is a trademark of Sigma-Aldrich Co. LLC

PLA is a registered trademark of Sigma-Aldrich Co. LLC

Texas Red is a registered trademark of Life Technologies

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