Duolink™ In Situ Orange Starter Kit Goat/Rabbit
Duolink™proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.[1][2]
Follow
the Duolink™ In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.
Visit our Duolink™ PLA Resource Center for information on how to run a Duolink™ experiment, applications, troubleshooting, and more.
To perform a complete Duolink™ PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This
starter kit supplies all other necessary reagents for 30 Duolink™ PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), orange detection reagents, wash buffers, and mounting medium. Note that the primary
antibodies must come from the same species as the Duolink™ PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink
™ PLA in situ reagents
are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink™ projects
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• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special
equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results
Duolink is a trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC
