Trizma® hydrochloride solution 1 M, BioReagent, for molecular biology
Trizma® hydrochloride solution is a useful biological buffer.
Trizma® hydrochloride solution may be used in the following studies:
• As buffer for the 2-D electrophoresis of rat fibroblast cell.[1]
• As buffer for the rapid isolation of high molecular
weight plant DNA (50,000 base pairs or more in length).[2]
• Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.[3]
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully
calibrated pH meter with a glass/calomel combination electrode.
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
