Branched Chain Amino Acid Kit sufficient for 100 colorimetric tests
General description
The branched-chain amino acids (BCAA), leucine, isoleucine, and valine, are the most common essential amino acids in proteins. The BCAA may function in both energy production and as nutrient signals.[1] Dietary supplementation with BCAA may aid in the treatment of various disorders such as Amyotrophic Lateral Sclerosis (ALS), latent and chronic hepatic encephalopathies,[2] and to prevent muscle atrophy in cancer patients. BCAA may increase the rate of protein synthesis and sensitize cells to insulin and insulin-like growth factor.[3]
Application
Branched Chain Amino Acid Kit has been used to determine the concentration of hepatic branched-chain amino acids, in a study assessing the role of CREB regulated transcription coactivator 2 (CRTC2) in hepatic lipid homeostasis and obesity in mice.[4]
Suitability
Suitable for measuring Branched Chain Amino Acids (BCAA’s) in a variety of biological samples.
Principle
In this assay, BCAA concentration is determined using a coupled enzyme reaction, which results in a colorimetric (450 nm) product, proportional to the BCAA present. The BCAA Assay Kit provides a simple convenient means of measuring the BCAAs in a variety of biological samples.
More detail
