Glutamine and Glutamate Determination Kit

Stock code :GLN1
Brand :Sigma-Aldrich
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Glutamine and Glutamate Determination Kit

Application

For enzymatic determination of L-glutamine and L-glutamate via reduction of NAD to NADH, measured spectrophotometrically at 339 nm.
The detection ranges for this kit are: 0.056 - 0.56 mM L-glutamate; 0.14 - 1.4 mM L-glutamine.

Glutamine and Glutamate Determination Kit has been used to determine the concentration of L-glutamine:
• in minimum essential medium (MEM) medium[1]
•  in the supernatant[2]
• in plasma[3]

Biochem/physiol Actions

The Glutamine/Glutamate Determination Kit is designed for the spectrophotometric measurement of L-glutamine and/or L-glutamate via enzymatic deamination of L-glutamine and dehydrogenation of L-glutamate with conversion of NAD+ to NADH. This kit may be used for the spectrophotometric measurement of L-glutamine and/or L-glutamate in liquid preparations.

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