Choline/Acetylcholine Quantification Kit sufficient for 100 colorimetric or fluorometric tests
Choline is an essential nutrient, commonly grouped with the B complex vitamins, that plays key roles in many biological processes. Choline is a precursor for the synthesis of acetylcholine, a critical neurotransmitter in both the peripheral and central nervous systems.
Choline/Acetylcholine Quantification Kit has been used to quantify the amounts of choline and acetylcholine in samples.[2][1]
Suitable for use with cell and tissue culture supernatants as well as serum, plasma, urine, and other biological fluids.
In this assay, choline concentration is determined by a coupled enzyme reaction, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the choline present. Acetylcholine levels can be determined by adding acetylcholinesterase to the reaction, which hydrolyzes acetylcholine to choline and acetate.
