Glycerol-3-Phosphate Assay Kit Sufficient

for 100 Colorimetric tests

Stock code :MAK207
Brand :Sigma-Aldrich
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Glycerol-3-Phosphate Assay Kit Sufficient

General description

Glycerol 3-phosphate (G3P) is an important intermediate of carbohydrate and lipid metabolic pathways. It is produced from glycerol by glycerol kinase or from dihydroxyacetone phosphate by glycerol 3-phosphate dehydrogenase. G3P may enter the G3P shuttle to generate NAD+, or may be converted to glyceraldehyde 3-phosphate and enter glycolysis or the lipid biosynthesis pathway.

Application

Glycerol-3-Phosphate Assay Kit has been used in the determination of glycerol-3-phosphate and phosphoenolpyruvate levels.[1] It has also been used to quantify the concentration in aerial mycelium.[2]

Suitability

Suitable for the detection of Glycerol-3-Phosphate in tissue and cell culture samples.

Principle

The Glycerol 3-Phosphate Colorimetric Assay Kit provides a simple assay for measuring G3P in various tissues and cells (ranging from 2-10 nmole/well). G3P is determined by measuring a colorimetric product with absorbance at 450 nm (A450) proportional to the amount of G3P present.

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