Oxaloacetate Assay Kit sufficient
Oxaloacetate (OAA) is an intermediate of both the TCA cycle and gluconeogenesis. It precedes citrate which is formed by the citrate synthase-mediated condensation of acetyl-CoA and OAA. OAA is also formed by the deamidation of aspartate or condensation of CO2 with pyruvate or PEP. Since mammals do not possess the enzymatic machinery to form TCA cycle intermediates from acetyl-CoA, OAA is one of the anaplerotic entry points via pyruvate and pyruvate carboxykinase. The Oxaloacetate Assay Kit provides a simple, sensitive and rapid means of quantifying OAA in a variety of samples.
Oxaloacetate Assay Kit has been used to determine the concentration of oxaloacetate in samples.[1]
Suitable for animal tissues, cell culture (adherent or suspension cells), serum and saliva.
In this kit, oxaloacetate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the oxaloacetate present. Typical detection ranges for this kit are 2–10 nmole (colorimetric) and 0.2–1 nmole (fluorometric).
