2-Phosphoglycerate Assay Kit Sufficient
2-Phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway, a critical mechanism of ATP generation. It is converted to phosphoenol pyruvate (PEP), catalyzed by the enzyme enolase. The consumption of glucose and the rate of glycolysis is upregulated in metastatic cancers, a phenomenon known as the Warburg effect. Measurement of intracellular levels of 2PG is a tool for analyzing the glycolytic pathway in cancer cells.
Suitable for the detection of 2-Phosphoglycerate in tissue and cell culture samples.
The 2-Phosphoglycerate Colorimetric/Fluorometric Assay Kit is a simple assay that measures 2PG in various samples (ranging from 2-10 nmole/well for the colorimetric assay and from 50-250 pmole/well for the fluorometric assay). 2PG concentration is determined by converting the 2PG to PEP and then to pyruvate. The pyruvate is oxidized, resulting in a colorimetric (570 nm) or fluorometric (λex = 535/ λem = 587 nm) product proportional to the amount of 2PG present.
The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.
