Xanthine/Hypoxanthine Assay Kit Sufficient
Xanthine is a purine base present in body fluids, tissues, and plants cells. It is also found in urinary calculi. Xanthine is formed by the oxidation of hypoxanthine during the degradation of adenosine monophosphate (AMP) to uric acid. Xanthine/ hypoxanthine
measurements in plasma and urine have been used to monitor allopurinol therapy. Cerebrospinal fluid (CSF) xanthine/hypoxanthine levels have been used as therapeutic guides and disease progression markers in hydrocephalus.
The Xanthine/Hypoxanthine
Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine
concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product, proportional to the xanthine/hypoxanthine present.
Note: The fluorometric assay
is ~10 times more sensitive than the colorimetric assay.
Compatible with high-throughput liquid handling systems.
Suitable for the detection of Xanthine/Hypoxanthine in a variety of samples such as tissue, cells, and body fluids.
The Xanthine/Hypoxanthine Assay Kit is a highly sensitive assay for determining xanthine/hypoxanthine levels (ranging from 4-20 nmole/well for the colorimetric assay and 40-200 pmole/well for the fluorometric assay) in a variety of biological samples. Xanthine/hypoxanthine concentration is determined by an enzymatic assay, which results in a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product, proportional to the xanthine/hypoxanthine present.
The fluorometric assay is ~10 times more sensitive than the colorimetric assay.
