Glucose-6-Phosphate Dehydrogenase Activity Assay Kit sufficient for 100

colorimetric tests

Stock code :MAK015

Brand :Sigma-Aldrich

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Glucose-6-Phosphate Dehydrogenase Activity Assay Kit sufficient for 100

General description

Glucose-6-phosphate dehydrogenase (G6PDH) catalyzes the conversion of glucose-6-phosphate to 6-phosphoglucono-δ-lactone, the first and rate-limiting step of the pentose phosphate pathway (PPP).^[1] The PPP pathway critical for maintaining the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH) and for the production of pentose sugars.^[2] The NADPH produced is critical for redox regulation via the regeneration of GSH and for providing reducing equivalents for fatty acid biosynthesis.^[4] Deficiencies in G6PDH predisposes individuals to non-immune hemolytic anemia.^[3]

Application

Glucose-6-phosphate dehydrogenase assay kit has been used in the determination of glucose-6-phosphate dehydrogenase (G6PDH) activity^[5]^[6] and to measure PDH (pyruvate dehydrogenase).^[7]

Suitability

Suitable for the measurement of Glucose-6-phosphate dehydrogenase (G6PDH) activity in various tissues and cells.

Principle

In the glucose-6-phosphate dehydrogenase assay kit, glucose-6-phosphate is oxidized to generate a product which is specifically detected by colorimetric (450 nm) assay. The G6PDH Assay Kit can detect G6PDH activity as low as 0.04mU G6PDH per well.

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