α−Mannosidase Assay Kit sufficient for 100 colorimetric tests
α-Mannosidase (AMA) is an enzyme which catalyzes the cleavage of the alpha form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children.
Compatible with high-throughput handling systems.
Suitable for determining α-Mannosidase (AMA) activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media.
The α-Mannosidase Activity Assay Kit provides a simple and high-throughput adaptable assay for determining α-Mannosidase activity in a variety of biological samples such as plasma, serum, tissue, and cell culture media. In this assay 4-nitrophenol is
cleaved from a synthetic substrate. Nitrophenol becomes intensely colored with the addition of the stop reagent, producing a colorimetric (405 nm) result, directly proportional to the enzyme activity in the sample.
One unit of AMA is the amount
of enzyme that will convert 1.0 μmole of 4-Nitrophenyl-α-
