Lambda DNA EcoR I Digest

Stock code :D5282
Brand :Sigma-Aldrich
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Lambda DNA EcoR I Digest

General description

Sigma’s Lambda DNA EcoR I Digest contains 6 fragments from 3,530 – 21,226 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Components

Sigma’s Lambda DNA EcoR I Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

Contains 6 fragments, 3,530–21,226 bp

For optimal resolution, the recommended agarose gel concentration is 0.7%.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I to completion, then purified by phenol extraction.

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