Titan One Tube RT-PCR System
The Titan™ One Tube RT-PCR System uses Reverse Transcriptase (RT) Avian Myeloblastosis Virus (AMV) for first strand cDNA synthesis and the Expand™ High Fidelity enzyme blend consisting of Taq DNA Polymerase and a polymerase with
a proofreading activity for amplification of cDNA by polymerase chain reaction (PCR). The proofreading activity, leads to an increased PCR fidelity. The Titan One Tube RT-PCR System:
• Reduces the error rate in PCR due to the proofreading
capability. A threefold higher fidelity is obtained in comparision to Taq DNA polymerase.
• Delivers increased sensitivity due to the high efficiency of all three enzymes.
• Is faster than conventional two-step RT-PCR.
Specificity of the reaction strongly depends on primer design and DNA contamination.
To exclude artefacts from DNA targets appropriate positive and negative control reaction should be included.
Heat inactivation: 2 min at 94 °C
Reverse
Transcriptase AMV
Titan™ One Tube RT-PCR System has been used:
• to amplify the regions containing the miRNA target (miRT) inserts/response elements in viral RNA[2][1]
• for determining
the presence or absence of RNA templates
• in cloning of rare messages without the need to construct cDNA libraries
• for quantifying the levels of gene expression
Titan™ One Tube RT-PCR System:
• High Fidelity: Expand™ High Fidelity enzyme blend with proofreading activity reduces the error rate in polymerase chain reaction (PCR)
• offers a threefold higher fidelity
in comparison to Taq DNA polymerase
• delivers increased sensitivity due to the high efficiency of all three enzymes
• faster than conventional two-step reverse transcriptase (RT)-PCR
• allows the amplification of full-length
cDNA up to 6 kb by RT-PCR due to the use of the Expand™ PCR System
1 kit containing 4 components
