GenElute™ 96 Well Tissue Genomic DNA Purification Kit (96-well x 2)
GenElute 96 Well Tissue Genomic DNA Purification Kit allows for high throughput purification of pure genomic DNA from a variety of cultured cells and tissues. The kit contains the advantages of silica membrane technology and eliminates the need for expensive resins, alcohol precipitations and hazardous compounds such as phenol and chloroform.
The purified genomic DNA is ready for use in downstream applications such as:
• PCR
• Southern blots
• cloning
• restriction digest
• enzymatic reactions
• Starting material: ≤ 20 mg tissue total (mouse, rat or whole sample) or ≤ 1 X 106 cultured cells or bacteria per well
• Average Expected yield: 10 - 20 μg/well (bacteria: 1.5 - 2.5 μg/well)
• Elution volume: 100 μl
• Time
required: 70 min/plate
• Innovative wash plate minimizes risk of cross-contamination
• Processing possible under vacuum or centrifugation
• Suitable for manual and automated processing
The starting materials are lysed in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are added to further promote solubilization of macromolecules. The next step is to add ethanol, which ensures that the appropriate binding conditions are created to bind the DNA to the silica membrane. Once the DNA is bound to the silica membrane, several wash buffers are used to remove contaminants. The final step is to elute pure genomic DNA in the Tissue Elution Buffer included in the kit.
GenElute is a trademark of Sigma-Aldrich Co. LLC
