GenElute™ Blood Genomic DNA Kit (70 preps)
The GenElute Blood Genomic DNA kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silica binding with a microspin format, and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.
GenElute™ Blood Genomic DNA Kit has been used:
• to extract DNA from samples of large granules (LG) and smaller granules (SG) obtained from vasectomized rabbits[2]
• to isolate DNA from
the blood samples [4]
• to extract whole blood DNA from the left blood cells[1]
The purified genomic DNA is ready for downstream applications such as:
• restriction endonuclease digestions
• PCR
• Southern blots
• sequencing reactions
• cloning
• Starting material: Up to 200 μl of fresh or aged blood
• Expected yield: Up to 10 μg
• Elution volume: 400 μl
• Time required: <40 min
• A260/A280 ratio: 1.6 - 1.9
• Compatible
with many anticoagulants, including EDTA, Heparin, and Sodium Citrate
The starting material is lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. The addition of ethanol causes the DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube.
A Prewash Solution is provided to help remove contaminants that are associated with aged (older than 24 hours) whole blood samples. After washing to remove contaminants, the DNA is eluted in 200 mL of a Tris-EDTA solution.
The expected
yields of genomic DNA will vary depending on the amount and nature of the starting material used (for example, 4 to 10 μg of RNase A-treated DNA can be isolated from 200 μl of fresh whole blood in less than one hour). DNA purified with this kit has
an A260/A280 ratio between 1.6 and 1.9 and can be up to 50 kb in length.
For additional information, please see www.sigma-aldrich.com/gen
GenElute is a trademark of Sigma-Aldrich Co. LLC
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