AXIS™ Axon Isolation Device, 900 µm
AXIS™ Axon Isolation Device, 900 µm |
Overview |
AXIS™ Axon Isolation Devices are tissue culture ready (TC) microfluidic tools that out-of-the-box enable researchers to easily isolate, observe, and test developing neurites. They are currently being offered in four different formats to provide test
flexibility during investigation.
AX90010 consists of 10 units of AX900, an axon isolation device consisting of four wells, two channels, and a set of microgrooves. Two of the wells are interconnected by a channel on each side of the device.
The microgrooves are located in the area between the two channels and their length is 900µm. Each microgroove is approximately 5µm in height by 10µm in width and are designed to allow neurite passage to traverse through while preventing cell bodies
from flowing across the device. There are roughly 120 microgrooves in each AX900 device.
The AXIS Axon Isolation Devices have been shown to effectively isolate cell bodies from differentiating neurites for a variety of cell types (Taylor
et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009) and will likely work with most neuronal cell type that can be successfully grown in tissue culture. Because the AXIS devices are made from an inert and
non-toxic polymer, cultures grown within AXIS devices can be maintained for weeks at a time. (Chun et al., 1992; Shibayama et al., 1991). In addition, because the polymer used to fabricate the axon isolation devices is optically clear, cells cultured
within the device can be imaged with high resolution microscopy, including live cell imaging, confocal, and differential interference microscopy (Taylor et al., 2005; Park et al., 2006; Liu et al., 2008; Yang, et al., 2009; Taylor et al., 2009).
The
microfluidic design of the AXIS devices is highly conducive to generation of hydrostatic pressure, which can be selectively used to isolate a solution, as well as chemical targets of interest, on a designated side of the device (Park et al., 2006).
In addition, immunocytochemistry (IHC) can also be performed within an AXIS Axon Isolation device and a protocol to do so is provided in the manual. |
More detail