SMC™ Human Phospho-AKT1 (pSer473) Immunoassay Kit
The SMC™ pAKT1 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
The serine-threonine protein kinase AKT family is the primary downstream mediator of the phosphatidylinositol-3-ki
Research Category
Inflammation & Immunology
The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human Phospho-AKT1 Immunoassay Kit unparalleled performance in quantifying pAKT1 in cell lysates.
PHOSPHO AKT1 Coated Beads
Sample Diluent
PHOSPHO AKT1 Detection Antibody
Assay Buffer
AKT1 Standard
AKT1 Detection Antibody Buffer
10X Wash Buffer
Elution Buffer B
Buffer D
SMC™ PHOSPHO AKT1 Immunoasay
Kit Instructions
Low-end CV% Average: 8%|Low-end CV% Range: 2 - 15%
SMC is a trademark of Singulex
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial