SMC™ Human Interferon-gamma (IFN-γ) Immunoassay Kit
Interferon-gamma (IFN-γ) is a cytokine with anti-viral and tumor-suppressing properties that is actively involved in the innate immune system. As the only member of the Type 2 IFN family, IFN-γ is expressed by Th1 cells, Tc cells, dendritic cells, and natural killer cells. Once bound to its heterodimeric receptor IFN-γR, the activated glycoprotein recruits and directs macrophages to the site of infection and escalates the inflammatory response. IFN-γ has also been documented to up-regulate the expression of IL-1 and down-regulate the production of IL-8, both of which are pro-inflammatory cytokines. The potency, specificity and rapid action of IFN-γ make it an attractive drug target for immuno-regulatory diseases.
The SMC™ Human IFN-γ Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human IFN-γ Immunoassay Kit unparalleled performance in the quantification of IFN-γ in human EDTA plasma.
Used to detect/quantify:IFN-γ
IFN-γ Coated Beads
Standard Diluent
IFN-γ Detection Antibody
Assay Buffer
Human IFN-γ Standard
SMC™ Human IFN-γ Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D
Low-end CV% Range: 1 - 12%|Low-end CV% Average: 8%
SMC is a trademark of Singulex
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
