SMC™ Human Interleukin 13 (IL-13) Immunoassay Kit
Interleukin-13 (IL-13) is a cytokine that is crucial for mediating allergic responses. Predominately expressed by Th2 cells, IL-13 is also secreted by cell types such as mast cells, NK cells, and dentritic cells. Both IL-13 and IL-4 are found at elevated levels in the asthmatic lung and are thought to be key regulators of airway inflammation. IL-13 functions similarly to IL-4 in that both can signal through the heterodimer receptor IL-4Rα - IL-13Rα1 and activate STAT-6 downstream. However, studies have suggested that IL-13 operates independently of IL-4 with regard to asthma regulation. Thus, IL-13 has become a promising therapeutic target for allergic diseases. Several anti-IL-13 antibodies are being evaluated as treatment for bronchial asthma. Predominately expressed by Th2 cells, IL-13 is also secreted by cell types such as mast cells, NK cells, and dentritic cells.
The SMC™ Human IL-13 Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured with a proprietary paramagnetic microparticle solid phase capture for ultimate biomarker quantification. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ IL-13 Immunoassay Kit unparalleled performance in the quantification of IL-13 in human EDTA plasma and serum.
Used to detect/quantify:IL-13
Assay Buffer
IL-13 Coated Beads (V2)
Standard Diluent
IL-13 Detection Antibody (V2)
Human IL-13 Standard
SMC™ Human IL-13 (V2) Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D
Low-end CV% Range: 0 - 12%|Low-end CV% Average: 6%
SMC is a trademark of Singulex
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
