Protease Inhibitor Cocktail Powder Reagents designed
Inhibits serine, cysteine, and metallo-proteases.
Optimized and tested for general use.
Protease inhibitor cocktail powder has been used in immunoblotting and immunoprecipitation.[2]
This mixture contains individual components, including AEBSF at 2 mM, Aprotinin at 0.3 μM, Bestatin at 116 μM, E-64 at 14 μM, Leupeptin at 1 μM and EDTA at 1 mM. Each component has specific inhibitory properties.
AEBSF and Aprotinin act to
inhibit serine proteases, including trypsin, chymotrypsin, and plasmin amongst others. Bestatin inhibits aminpeptidases. E-64 acts against cystein proteases. EDTA is an inhibitor of metalloproteases. Leupeptin acts against both serine and cystein
proteases.
Protease helps to break down proteins into soluble peptides and amino acids.[1]
1 bottle in serum bottle
Components
AEBSF
Aprotinin
Bestatin
E-64
EDTA
Leupeptin
One bottle makes 100 mL of cocktail, which are sufficient to the inhibition of proteases present in approximately 20 gram of cell extract.
Not all extracts contain the same levels of endogenous enzymes, and it may be necessary to adjust the volume
of cocktail required.
This product is supplied as a lyophilized powder. One bottle prepares 100 mL of a cocktail solution.
