Duolink™ In Situ Microplate Nuclear Stain, Anti-Fade
Duolink™proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.
Use
the Multiwell Plates modifications to the Duolink™ In Situ Fluorescence Protocol to run an experiment with this product. A set of short instructions can also be used.
Visit our Duolink™ PLA Resource Center for information on how to run a Duolink™ experiment, applications, troubleshooting, and more.
To perform a complete Duolink™ PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same
species as the Duolink™ PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
Duolink™ In Situ Microplate Nuclear Stain and Anti-Fade are intended to be used
after staining cells with Duolink™ In Situ in microtiter plates. See the datasheet for more information.
Application Note
Two primary antibodies raised in different species are needed. Test your primary antibodies (IgG-class,
mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC) or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink™ in situ reagents are suitable for
use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.
Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink™ projects
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• No overexpression or genetic manipulation required
• High specificity (fewer false positives)
• Single molecule sensitivity due to rolling circle amplification
• Relative quantification possible
• No special
equipment needed
• Quicker and simpler than FRET
• Increased accuracy compared to co-IP
• Publication-ready results
Duolink is a trademark of Sigma-Aldrich Co. LLC
PLA is a registered trademark of Sigma-Aldrich Co. LLC
