SSPE Buffer 20× Concentrate for Northern and Southern blotting, solution
Trizma® hydrochloride solution is a useful biological buffer.
Trizma® hydrochloride along with Trizma-base is used to prepare buffer solutions of pH 7.35.[2] It was used to prepare the triton extract.[4] It may be used in the analysis of protease activity, isolated from the Bacillus stearothermophilus and Bacillus subtilis.[1] It may be employed as buffer for the restriction fragment analysis of genomic DNA isolated from the enterococcal isolates and Escherichia coli.[3]
The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully
calibrated pH meter with a glass/calomel combination electrode.
Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
