Dimethyl sulfoxide PCR Reagent
Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs).[1] Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination.[2] As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.[3]
Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture[4] and as PCR facilitator.[2][5]
Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table
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