MgCl2 Stock Solution
The MgCl2 Stock Solution is used for the individual adjustment of the Mg2+-concentration in the PCR reaction and can be used in combination with the PCR buffer without MgCl2. In most applications a concentration of 1.5mM MgCl2 will yield satisfactory results with a dNTP concentration of 200μM each. In many cases, however, it is required to titrate the optimal Mg2+ concentration to increase the specificity and the yield of the PCR reaction.
The combination of PCR Buffer without MgCl2 and the MgCl2 Stock Solution buffer are optimized for the amplification of specific DNA fragments in conventional PCRs (i.e., with Taq DNA Polymerase). However, the MgCl2 Stock Solution may be used with other, more appropriate PCR buffers to optimize any type of PCR (e.g., hot start PCR, high fidelity PCR, long template PCR).
Magnesium (Mg2+) chelates nucleotide triphosphates (dNTPs) and its usage is one among the optimization strategies for polymerase chain reaction (PCR).[1] It promotes orthophosphate synthesis from oligophosphates by stabilizing the phosphate groups of nucleotides.[2]
The MgCl2 Stock Solution contains 25mM MgCl2 in sterile water.
For life science research only. Not for use in diagnostic procedures.
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