Poly-L-lysine hydrobromide mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable
Poly-
Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
Molecular weight based on viscosity. Also assayed by MALLS.
For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.
