SMC™ Human Total Glucagon-Like Peptide-1
Glucagon-Like Peptide-1 (GLP-1) is a member of the incretin family of hormones, which are released by the gut into the bloodstream in response to food. Post-translational cleavage of the peptide produces active GLP-1, which is then rapidly degraded by protease dipeptidyl peptidase-4 (DPP-4). When blood glucose level is high, active GLP-1 enhances insulin secretion and also suppresses the release of glucagon. In addition, GLP-1 stimulates the brain to trigger feelings of satiety and regulates the rate of gastric emptying. Compared to healthy controls, some patients with type-2 diabetes show a modest decrease in GLP-1 release. Therapeutic GLP-1 analogs such as exenatide, or DPP inhibitors like sitagliptin, are promising strategies for treating type-2 diabetes.
The SMC™ Total GLP-1 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Research Category
Metabolism
Research Sub Category
Metabolic Disorders
The sensitivity of Single Molecule Counting (SMC™) technology gives the Total GLP-1 Immunoassay Kit unparalleled performance in the quantification of total GLP-1 in human EDTA plasma.
Used to detect/quantify:GLP-1 (Total)
Total GLP-1 Coated Beads Vial 1
Total GLP-1 Coated Beads Vial 2
Total GLP-1 Standard Diluent
GLP-1 Assay Buffer
Total GLP-1 Standard
Total GLP-1 Detection Antibody
SMC™Total GLP-1 Immunoassay Kit Instructions
10X Wash
Buffer
Elution Buffer B
Buffer D
Low-end CV% Range: 5 - 12%|Low-end CV% Average: 9%
SMC is a trademark of Singulex
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
