SMC™ Human Interleukin 4 (IL-4) Immunoassay Kit
Interleukin-4 (IL-4), is a cytokine that is a key regulator in humoral and adaptive immunity. IL-4 induces differentiation of naive helper T-cells (Th0 cells) to Th2 cells. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. Further, IL-4 controls the production of IgE, expands IL-4 producing T-cell subsets, and stabilizes effector cell functions. IL-4 has great therapeutic potential due to its role in the development of allergic inflammatory responses. IL-4 also has shown to have promise in drug targeting for cancer.
The SMC™ Human IL-4 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC™) gives the SMC™ Human IL-4 Immunoassay Kit unparalleled performance in the quantification of IL-4 in human EDTA plasma.
Used to detect/quantify:IL-4
IL-4 Coated Beads
Standard Diluent
IL-4 Detection Antibody
Assay Buffer
IL-4 Standard
SMC™ Human IL-4 Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D
Low-end CV% Range: 2 - 12%|Low-end CV% Average: 7%
SMC is a trademark of Singulex
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial