SMC™ Human Interleukin 5 (IL-5) Immunoassay Kit
General description
Interleukin-5 (IL-5) is a cytokine secreted mainly by T-cells and mast cells upon stimulation with antigens and allergens. It has pleiotropic effects toward B-cells and is involved in the production of immunoglobulins. More importantly, IL-5 acts in synergy with GM-CSF to govern the proliferation and maturation of eosinophils. A pronounced level of eosinophils is commonly associated with the development of allergic conditions such as asthma, eosinophilic esophagitis and hypereosinophilic syndrome. As such, IL-5 is highly regarded as a crucial mediator of the inflammatory pathway concerning allergic reactions. Current studies suggest that IL-5 blockade is a promising intervention for the treatment of allergic diseases.
The SMC™ Human IL-5 Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured with a proprietary paramagnetic microparticle solid phase capture for ultimate biomarker quantification. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Application
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule County (SMC™) gives the SMC™ Human IL-5 Immunoassay Kit unparalleled performance in the quantification of IL-5 in human EDTA plasma.
Used to detect/quantify:IL-5
Components
IL-5 Coated Beads
Standard Diluent
IL-5 Detection Antibody
Assay Buffer
IL-5 Standard
SMC™ IL-5 Immunoassay Kit Instructions
10X Wash Buffer
Elution Buffer B
Buffer D
Legal Information
SMC is a trademark of Singulex
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
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