SMC™ Human Interleukin 6 (IL-6) Immunoassay Kit
Interleukin-6 (IL-6) is a pro-inflammatory cytokine secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation. IL-6 is also secreted by macrophages in response to specific microbial molecules, referred to as pathogen associated molecular patterns (PAMPs), which trigger the innate immune response and initiate inflammatory cytokine production. IL-6 is one of the most important mediators of fever and of the acute phase response. IL-6 is also called a "myokine", a cytokine produced from muscle that increases in response to muscle contraction. Additionally, osteoblasts secrete IL-6 to stimulate osteoclast formation.
The SMC™ IL-6 Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC™ immunoassay system detects analytes using Single Molecule Counting (SMC™) technology.
Research Category
Inflammation & Immunology
Research Sub Category
Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-6 Immunoassay Kit unparalleled performance in quantification of IL-6 in human EDTA plasma.
Used to detect/quantify:IL-6
Assay Buffer
IL-6 Coated Beads
Human IL-6 Standard Diluent
IL-6 Detection Antibody
Human IL-6 Standard
10X Wash Buffer
Buffer D
Elution Buffer B
SMC™ Human IL-6 Immunoassay Kit Instructions
Low-end CV% Range: 2 - 8%|Low-end CV% Average: 6%
SMC is a trademark of Singulex
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
