HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture
HEPES has been described as one of the best all-purpose buffers available for biological research.[1] At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.[4]
HEPES has been used as a component of:
• Hank’s balanced salt solution, Dulbecco’s modified eagle’s medium and no-glucose DMEM, which are used for the preparation of tissue slices[2][3]
• Buffers
used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity[3]
Concentrations of HEPES over 40mM may be toxic to some cell types.
Prepared from HEPES free acid and cell culture grade water.
