Trizma Base
40% (w/w) solution in water is clear and colorless.
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[2] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[3] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[13]
Trizma® base has been used:
• as a component of H buffer (cell dissociation buffer)[10]
• for washing and saturation of wells in double sandwich ELISA immunoenzymatic technique[4]
• as
an assay buffer for reconstitution of extracted and dried protein samples[5]
• to prepare Tris-HCl buffer that is used to stabilize proteins[6]
• as a buffer to extract
carotenoid from tubers[1]
• as a component of sample buffer during protein extraction prior to western blotting[12]
• as a component of sample buffer for sodium dodecyl
sulfate polyacrylamide gel electrophoresis (SDS-PAGE)[15]
• to prepare simulated body fluid (SBF) for calcium phosphate (CaP) resorption assay[14]
• as a buffer for polydopamine
(PDA) deposition on stainless steel (SS) substrate
1 kg in poly bottle
25, 100, 250, 500 g in poly bottle
5, 10, 25, 50 kg in poly drum
